Asian Journal of Bio Science

Anjali Tiwari ¹, O. P. Verma ¹, Manishi Tripathi ²

Affiliations
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, ALLAHABAD (U.P.), IN
2 Department of Microbiology, IBSBT, CSJM University, KANPUR (U.P.), IN

Abstract

Resistance and tolerance are arbitrarily defined terms frequently interchangeable and after based on weather a given isolate can grow in the presence of selected heavy metal concentration in laboratory media. Total bacterial strains were isolated and screened on the basis of their heavy metal tolerance ability for cadmium and their PGPR activity. It was estimated that they have good tolerance power for cadmium the heavy metals and are plant growth promoting rhizobacteria. If we study morphological aspects we can say that Gram negative rods are more efficient in tolerating heavy metals. In case of treatment I, fresh weight was increased 27.5 per cent and dry weight was increased 25 per cent compared to positive control. In case of treatment II, fresh weight was increased 28 per cent and dry weight was increased 14.81 per cent compared to negative control. In case of negative control, fresh weight was decreased 50 per cent and dry weight was decreased 48.07 per cent compared to positive control. By performing experiment it was proved that the cadmium concentration reduced significant percentage of Chaulai fresh weight and dry weight but increased when treated with strain RAN 1.

Keywords

Rhizobacteria, Tolerance, Resistance, Functional Characterization, Cadmimum.

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Anjali Tiwari ¹, O. P. Verma ¹, Manishi Tripathi ²

Affiliations
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
2 Department of Microbiology, Institute of Biosciences and Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur (U.P.), IN

Abstract

Resistance and tolerance are arbitrarily defined terms frequently interchangeable and after based on weather a given isolate can grow in the presence of selected heavy metal concentration in laboratory media. Total bacterial strains were isolated and screened on the basis of their heavy metal tolerance ability for cadmium and their PGPR activity. It was estimated that they have good tolerance power for cadmium the heavy metals and are plant growth promoting rhizobacteria. If we study morphological aspects we can say that Gram negative rods are more efficient in tolerating heavy metals. In case of treatment I, fresh weight was increased 22.22 per cent and dry weight was increased 20.73 per cent compared to positive control. In case of treatment II, fresh weight was increased 42 per cent and dry weight was increased 20 per cent compared to negative control. In case of negative control, fresh weight was decreased 56.52 per cent and dry weight was decreased 50 per cent compared to positive control. By performing experiment it was proved that the cadmium concentration reduced significant percentage of chaulai fresh weight and dry weight but increased when treated with strain RCP 1.

Keywords

Rhizobacteria, Tolerance, Resistance, Functional Characterization,Cadmium.

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Anjali Tiwari ¹, O. P. Verma ¹, Manishi Tripathi ²

Affiliations
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
2 Department of Microbiology, Institute of Biosciences and Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur (U.P.), IN

Abstract

Resistance and tolerance are arbitrarily defined terms frequently interchangeable and after based on weather a given isolate can grow in the presence of selected heavy metal concentration in laboratory media. Total bacterial strains were isolated and screened on the basis of their heavy metal tolerance ability for cadmium and their PGPR activity. It was estimated that they have good tolerance power for cadmium, the heavy metals and are plant growth promoting rhizobacteria. If we study morphological aspects we can say that Gram negative rods are more efficient in tolerating heavy metals. By performing different PGPR test it was proved that the isolated strains have PGPR activity can be used as biopesticides and biofertilizers. At pH 7 and 28⁰C temperature strain RCP 1 was resistant to heavy metal but at pH 9, the growth was inhibited in presence of heavy metal. There were high growth rate of strain RCP 1 at 28⁰C. Strain RCP 1 grew at neutral and alkaline pH also, but in presence of heavy metal growth was increased. In presence of heavy metal, strain RCP 1 increased the ischolar_main and shoot length as well as fresh and dry weight also. So, the microbes isolated from RCP 1 that must have good tolerance power and developed resistance mechanism for their survival and thus, can be helpful in bioremediation.

Keywords

Rhizobacteria, Tolerance, Resistance, PGPR, Cadmium.

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Anjali Tiwari ¹, Ankit Pandey ¹, O. P. Verma ¹

Affiliations
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN

Abstract

The use of plant based drugs and chemicals for curing various ailments and personal adornment is as old as human civilization. Plants and plant-based medicaments are the basis of many of the modern pharmaceuticals we use today for our various ailments. Methanolic extracts of dried leaves of Ocimum sanctum, Azadirachta indica and Phyllanthus emblica were used for the comparative study of phytochemical constituents. A qualitative phytochemical analysis was performed for the detection of alkaloids, glycosides, saponins, steroids, flavonoids, tannins and reducing sugar. The highest yield of methanolic extract was found in Azadirachta indica (29.08%). Ocimum sanctum contained all the chemicals except flavonoids and reducing sugar, however, the Colquhounia coccinea lacked alkaloids and reducing sugar.

Keywords

Phytochemical Screening, Alkaloids, Glycosides, Steroids, Flavonoids, Tannins, Reducing Sugar.

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